Characterization And Acute Toxicity Test Of Black Garlic Ethanol Exctract Based On OECD

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PDF    Published Jan 6, 2022    DOI      Abstract viewed: 135   PDF viewed: 47  
Ellen Stephanie Rumaseuw
Yoppi Iskandar
Eli Halimah
Ade Zuhrotun


Background: Garlic (Allium sativum, L) was used empirically by the ancestors as a useful plant for hypercholesterolemia. The processed garlic product, namely black garlic, has gone through an optimal heating process at a temperature of 70oC for 21 days. Black garlic can become a standardized herb if it has been through characterization and toxicity tests.

Methods: The characterization test is known by measuring several general standard parameters of Indonesian extracts. Acute toxicity test was carried out by dividing 5 treatment groups namely negative control, group I (5 mg/kg BW), group II (50 mg/kg BW), group III (300 mg/kg BW), group IV (2000 mg/kg BW).

Results: The results showed that the standardization of black garlic extract has a drying shrinkage content of 7.24%, a water content of 8.8%, a total ash content of 4.79%, an acid insoluble ash content of 1.52%, a water soluble extract content of 7.47% and an ethanol soluble extract content of 9.3% which is still into the Indonesian herbal pharmacopoeia standard and the administration of black garlic ethanol extract caused mild toxic symptoms during the acute toxicity test and obtained LD50 values > 2000 mg/kg BW.

Conclusion: Characterization of black garlic extract according to standardization parameters of Indonesian plant extracts and administration of ethanolic extract of black garlic has an LD50 value > 2000 mg/kg BW which is included in category 5 in the OECD.

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Rumaseuw, E., Iskandar, Y., Halimah, E., & Zuhrotun, A. (2022). Characterization And Acute Toxicity Test Of Black Garlic Ethanol Exctract Based On OECD. Interest : Jurnal Ilmu Kesehatan, 215-224.


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