Main Article Content
Background: Garlic (Allium sativum, L) was used empirically by the ancestors as a useful plant for hypercholesterolemia. The processed garlic product, namely black garlic, has gone through an optimal heating process at a temperature of 70oC for 21 days. Black garlic can become a standardized herb if it has been through characterization and toxicity tests.
Methods: The characterization test is known by measuring several general standard parameters of Indonesian extracts. Acute toxicity test was carried out by dividing 5 treatment groups namely negative control, group I (5 mg/kg BW), group II (50 mg/kg BW), group III (300 mg/kg BW), group IV (2000 mg/kg BW).
Results: The results showed that the standardization of black garlic extract has a drying shrinkage content of 7.24%, a water content of 8.8%, a total ash content of 4.79%, an acid insoluble ash content of 1.52%, a water soluble extract content of 7.47% and an ethanol soluble extract content of 9.3% which is still into the Indonesian herbal pharmacopoeia standard and the administration of black garlic ethanol extract caused mild toxic symptoms during the acute toxicity test and obtained LD50 values > 2000 mg/kg BW.
Conclusion: Characterization of black garlic extract according to standardization parameters of Indonesian plant extracts and administration of ethanolic extract of black garlic has an LD50 value > 2000 mg/kg BW which is included in category 5 in the OECD.
Co-operation, O. for E. & Development. (2002). Test No. 420: Acute Oral Toxicity-Fixed Dose Procedure. OECD Publishing. https://ntp.niehs.nih.gov/iccvam/suppdocs/feddocs/oecd/oecd_gl420.pdf
Dong, M., Yang, G., Liu, H., Liu, X., Lin, S., Sun, D., & Wang, Y. (2014). Aged black garlic extract inhibits HT29 colon cancer cell growth via the PI3K/Akt signaling pathway. Biomedical Reports, 2(2), 250–254. https://doi.org/10.3892/br.2014.226
Ha, A. W., Ying, T., & Kim, W. K. (2015). The effects of black garlic (Allium sativum) extracts on lipid metabolism in rats fed a high fat diet. Nutrition Research and Practice, 9(1), 30–36. https://doi.org/10.4162/nrp.2015.9.1.30
Jeong, Y. Y., Ryu, J. H., Shin, J.-H., Kang, M. J., Kang, J. R., Han, J., & Kang, D. (2016). Comparison of anti-oxidant and anti-inflammatory effects between fresh and aged black garlic extracts. Molecules, 21(4), 430. https://doi.org/10.3390/molecules21040430
Jung, E.-S., Park, S.-H., Choi, E.-K., Ryu, B.-H., Park, B.-H., Kim, D.-S., Kim, Y.-G., & Chae, S.-W. (2014). Reduction of blood lipid parameters by a 12-wk supplementation of aged black garlic: A randomized controlled trial. Nutrition, 30(9), 1034–1039. https://doi.org/10.10.2014.02.014
Kimura, S., Tung, Y.-C., Pan, M.-H., Su, N.-W., Lai, Y.-J., & Cheng, K.-C. (2017). Black garlic: A critical review of its production, bioactivity, and application. Journal of Food and Drug Analysis, 25(1), 62–70. https://doi.org/10.1016/j.jfda.2016.11.003
Marjoni, R. (2016). Dasar-dasar fitokimia untuk diploma III farmasi. Jakarta: Cv. Trans Info Media.
Miladulhaq, M. (2018). Perubahan Sifat Fisikokimia Selama Pengolahan Bawang Putih Tunggal Menjadi Bawang Hitam Menggunakan Rice Cooker. https://repository.ipb.ac.id/handle/123456789/95561
Nuristika, V. (2018). Uji Toksisitas Akut Bawang Hitam Lanang terhadap Mencit Jantan Galur Swiss Webster (Skripsi). Tangerang: STF Muhammadiyah. http://aa880a96537f.sn.mynetname.net/repository/gdl.php?mod=browse&op=read&id=gdl-20181022-000314---vidyanuris-20
RI, M. (2016). Peraturan Menteri Kesehatan Republik Indonesia Nomor 6 Tahun 2016 Tentang Formularium Obat Herbal Asli Indonesia. Kementerian Kesehatan Republik Indonesia. Jakarta.
Sasaki, J.-I. (2015). Overview of the black garlic movement in the fields of research and marketing. Journal of Life Sciences, 9, 65–74. http://www.davidpublisher.com/Public/uploads/Contribute/559f6c1fcd3a1.pdf
Wang, X., Jiao, F., Wang, Q.-W., Wang, J., Yang, K., Hu, R.-R., Liu, H.-C., Wang, H.-Y., & Wang, Y.-S. (2012). Aged black garlic extract induces inhibition of gastric cancer cell growth in vitro and in vivo. Molecular Medicine Reports, 5(1), 66–72. https://doi.org/10.3892/mmr.2011.588