Antibacterial Activity Of Combination Of n-Hexane Extract Of Buas-Buas Leaves (Premna serratifolia L.) And Sappan Wood (Caesalpinia sappan L.)
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Abstract
Background: The plants used in this study were buas-buas leaves (Premna serratifolia L.) and sappan wood (Caesalpinia sappan L.). The people of West Kalimantan use the buas-buas leaves and sappan wood as traditional medicine, and, based on research, they have antibacterial activity. Staphylococcus aureus and Escherichia coli are bacteria that cause nosocomial infections and have developed resistance to many antibiotics. This research was conducted to determine the antibacterial activity of the combination of n-hexane extract of buas-buas leaves and sappan wood.
Methods: The antibacterial activity test was carried out by the agar diffusion method using a paper disc with a diameter of 6 mm. The antibacterial activity of a combination of n-hexane extract of buas-buas leaves and sappan wood in a ratio of 1:1, 2:1, and 1:2 at concentrations of 125, 250, and 500 ppm.
Results: In this study, the combination of n-hexane extract of buas-buas leaves and sappan wood with various concentrations, even with increasing concentrations, did not show any inhibition zones. The factors that are thought to affect the absence of the inhibition zone are the concentration of the extract, the content of secondary metabolites, the solubility of the extract, and the length of contact time
Conclusion: The combination of n-hexane extract of buas-buas leaves and sappan wood did not have antibacterial activity against Staphylococcus aureus and Escherichia coli in all comparisons and concentrations. It is recommended to test the antibacterial activity with a higher concentration
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References
Afnidar. (2014). Fitokimia Dan Uji Aktivitas Antibakteri Ekstrak Kalus Tumbuhan Sernai (Wedelia biflora ( L ) DC .). Jesbio, 3(4), 9–16.
Agustin, B. A., Puspawaty, N., & Rukmana, R. M. (2018). Aktivitas Antibakteri Kombinasi Ekstrak Etanolik Daun Beluntas (Pluchaea indica Less.) dan Meniran (Phyllanthus niruri L.) terhadap Bakteri Staphylococcus aureus. Biomedika, 11(2), 79–87. https://doi.org/10.31001/biomedika.v11i2.425
Astuti, E., Sunarminingsih, R., Jenie, U. A., Mubarika, S., & Sismindari. (2014). Pengaruh lokasi tumbuh, umur tanaman dan variasi jenis destilasi terhadap komposisi senyawa minyak atsiri rimpang Curcuma mangga produksi beberapa sentra di Yogyakarta. Journal of People and Environment, 21(3), 323–330.
Baharutan, A., Rares, F. E. S., & Soeliongan, S. (2015). Pola Bakteri Penyebab Infeksi Nosokomial Pada Ruang Perawatan Intensif Anak Di Blu Rsup Prof. Dr. R. D. Kandou Manado. Jurnal E-Biomedik, 3(1). https://doi.org/10.35790/ebm.3.1.2015.7417
Baljeet, S. Y., Simmy, G., Ritika, Y., & Roshanlal, Y. (2015). Antimicrobial activity of individual and combined extracts of selected spices against some pathogenic and food spoilage microorganisms. International Food Research Journal, 22(6), 2594–2600.
Bukke, A. N., Hadi, F. N., & Produtur, C. S. (2015). Comparative study of in vitro antibacterial activity of leaves, bark, heart wood and seed extracts of Caesalpinia sappan L. Asian Pacific Journal of Tropical Disease, 5(11), 903–907. https://doi.org/10.1016/S2222-1808(15)60954-9
Cowan, M. M. (1999). Plant Products as Antimicrobial Agents. Clinical Microbiology Review, 12(4), 564–582.
Fitri, W. N., & Rahayu, D. (2018). Review: Aktivitas Antibakteri Ekstrak Tumbuhan Melastomataceae Terhadap Bakteri Escherichia coli Dan Staphylococcus aureus. Farmaka Suplemen, 16(3), 69–77.
Ghaneian, M. T., Ehrampoush, M. H., Jebali, A., Hekmatimoghaddam, S., & Mahmoudi, M. (2015). Antimicrobial activity, toxicity and stability of phytol as a novel surface disinfectant. Environmental Health Engineering and Management Journal, 2(1), 13–16.
Hadiarti, D. (2015). IDENTIFIKASI EKSTRAK n-HEKSANA SENYAWA BUAS-BUAS (Premna serratifolia Linn) MENGGUNAKAN GC-MS. Jurnal Buletin Al-Ribaath, 12(1), 22–28. https://doi.org/10.29406/br.v12i1.76
Hilal A. Syahrir, N., Mochamad Afendi, F., & Susetyo, B. (2016). Efek Sinergis Bahan Aktif Tanaman Obat Berbasiskan Jejaring dengan Protein Target. Jurnal Jamu Indonesia, 1(1), 35–46. https://doi.org/10.29244/jjidn.v1i1.30594
Huish, R. D., Huish, K. H., Walker, T. J., & Shrestha, G. (2014). Traditional Tongan treatments for infections: Bioassays and ethnobotanical leads for activity. Journal of Medicinal Plants Research, 8(41), 1215–1222. https://doi.org/10.5897/JMPR2014.5558
Ilhani, A. F. (2018). UJI AKTIVITAS ANTIBAKTERI KOMBINASI EKSTRAK ETANOL RIMPANG KENCUR ( Kaempferia galanga L ) DAN EKSTRAK ETANOL DAUN SAWO ( Manilkara zapota L ) PADA BAKTERI Escherichia coli. Jurnal Pelita Informatika, 7(1), 103–107.
Katrin, D., Idiawati, N., & Sitorus, B. (2015). Uji Aktivitas Antibakteri dari Ekstrak Daun Malek (Litsea graciae Vidal) terhadap Bakteri Stapylococcus aureus dan Escherichia coli. Jkk, 4(1), 7–12. Retrieved from https://jurnal.untan.ac.id/index.php/jkkmipa/article/view/11720/11003
Katuuk, R. H. H., Wanget, S. A., & Tumewu, P. (2019). Pengaruh Perbedaan Ketinggian Tempat Terhadap Kandungan Metabolit Sekunder Pada Gulma Babadotan (Ageratum Conyzoides L.). Cocos, 1(4).
Kristianti AN, Aminah NS, Tanjung M dan Kurniadi B. 2008. Buku Ajar Fitokimia. Surabaya: Jurusan Kimia Laboratorium Kimia Organik Fmipa Universitas Airlangga.
Kumesan, N., Yamlean, P. V. Y., & Supriati, H. S. (2013). Formulasi Dan Uji Aktivitas Gel Antijerawat Ekstrak Umbi Bakung (Crinum Asiaticum L.) Terhadap Bakteri Staphylococcus Aureus Secara in Vitro. PHARMACON Jurnal Ilmiah Farmasi – UNSRAT, 2(2), 2302–2493.
Kurniati, ruth indah. (2013). Kurniati, ruth indah. 2013. Uji Aktivitas Antioksidan Fraksi Etanol Daun Buas-Buas (Premna cordifolia Linn.) dengan Metode DPPH (2,2-difenil-1-pikrilhidrazil). Jurnal Mahasiswa Farmasi Fakultas Kedokteran UNTAN, 3(1), 1–13.
Lingga, A. R., Pato, U., & Rossi, E. (2015). Uji Antibakteri Ekstrak Batang Kecombrang (Nicolaia speciosa Horan) Terhadap Staphylococcus aureus Dan Escherichia coli. JOM Faperta, 2(2).
Minh, N. (2019). Different Factors affecting Dried Herbal Tea Production from Premna serratifolia Leaf. Journal of Pharmaceutical Sciences and Research, 11(3), 806–808.
Nirmal, N. P., Rajput, M. S., Prasad, R. G. S. V., & Ahmad, M. (2015). Brazilin from Caesalpinia sappan heartwood and its pharmacological activities: A review. Asian Pacific Journal of Tropical Medicine, 8(6), 421–430. https://doi.org/10.1016/j.apjtm.2015.05.014
Nomer, N. M. G. R., Duniaji, A. S., & Nocianitri, K. A. (2019). KANDUNGAN SENYAWA FLAVONOID DAN ANTOSIANIN EKSTRAK KAYU SECANG (Caesalpinia sappan L.) SERTA AKTIVITAS ANTIBAKTERI TERHADAP Vibrio cholerae. Jurnal Ilmu Dan Teknologi Pangan (ITEPA), 8(2), 216. https://doi.org/10.24843/itepa.2019.v08.i02.p12
Nuralifah, N., Jabbar, A., Parawansah, P., & Iko, R. A. (2018). Uji Toksisitas Akut Ekstrak Etanol Daun Notika (Archboldiodendron calosercium (Kobuski)) Terhadap Larva Artemia salina Leach dengan Menggunakan Metode Brine Shrimp Lethality Test (BSLT). Pharmauho: Jurnal Farmasi, Sains, Dan Kesehatan, 4(1), 1–5. https://doi.org/10.33772/pharmauho.v4i1.4618
Poeloengan, M., & Praptiwi. (2010). Uji Aktivitas Antibakteri Ekstrak Kulit Buah Manggis (Gardnia mangostana Linn). Media Litbang Kesehatan, 20(2), 65–69.
Prabawa, I. D. G. P., Khairiah, N., & Ihsan, H. K. (2019). Bioaktivitas Dan Metabolit Sekunder Dari Ekstrak Kayu Secang (Caesalpinia Sappan L.) Untuk Sediaan Bahan Aktif Study Of Bioactivity And Secondary Metabolites From Secang Wood Extract (Caesalpinia Sappan L.) As Active Ingredient. Prosiding Seminar Nasional Ke-2 Tahun 2019 Balai Riset Dan Standardisasi Industri Samarinda, 1–12.
Primadianti, A., Retnaningsih, A., & Ningrum, A. S. (2019). Aktivitas antimikroba kombinasi air perasan daun mengkudu (Morinda citrifolia L.) dan daun pepaya (Carica papaya L.) terhadap bakteri Escherichia coli dan Shigella dysentriae. Jurnal Analis Farmasi, 4(2), 130–138.
Rahman, A., Sultana Shanta, Z., Rashid, M. A., Parvin, T., Afrin, S., Khodeza Khatun, M., & Sattar, M. A. (2016). In vitro antibacterial properties of essential oil and organic extracts of Premna integrifolia Linn. Arabian Journal of Chemistry, 9(1), S475–S479. https://doi.org/10.1016/j.arabjc.2011.06.003
Raini, M. (2017). Antibiotik Golongan Fluorokuinolon: Manfaat dan Kerugian. Media Penelitian Dan Pengembangan Kesehatan, 26(3), 163–174. https://doi.org/10.22435/mpk.v26i3.4449.163-174
Rieuwpassa, I. E., Yunus, M., & Arsana, I. W. S. (2011). Identifikasi Pseudomonas aeruginosa dan tes sensitivitas siprofloksasin pada abses periodontal Identification of Pseudomonas aeruginosa and sensitivity test of ciprofloxacin on periodontal abcess. Journal of Dentomaxillofacial Science, 10(3), 151. https://doi.org/10.15562/jdmfs.v10i3.274
Septiani, S., Dewi, E. N., & Wijayanti, I. (2017). AKTIVITAS ANTIBAKTERI EKSTRAK LAMUN (Cymodocea rotundata) TERHADAP BAKTERI Staphylococcus aureus DAN Escherichia coli (Antibacterial Activities of Seagrass Extracts (Cymodocea rotundata) Against Staphylococcus aureus and Escherichia coli). SAINTEK PERIKANAN : Indonesian Journal of Fisheries Science and Technology, 13(1), 1. https://doi.org/10.14710/ijfst.13.1.1-6
Setyowati, W. A. E., & Damayanti, D. R. (2014). Pengaruh Metode Ekstraksi Terhadap Aktivitas Antioksidan Kulit Buah Durian (Durio zibethinus Murr) Varietas Petruk. SNPS (Seminar Nasional Pendidikan Sains), 1.
Siregar, A. F., Sabdono, A., & Pringgenies, D. (2012). Potensi Antibakteri Ekstrak Rumput Laut Terhadap Bakteri Penyakit Kulit Pseudomonas aeruginosa, Staphylococcus epidermidis, dan Micrococcus luteus. Diponegoro Journal of Marine Research, 1(2), 152–160.
Srinivasan, R., Selvam, G. G., Karthik, S., Mathivanan, K., Baskaran, R., Karthikeyan, M., … Govindasamy, C. (2012). In vitro antimicrobial activity of Caesalpinia sappan L. Asian Pacific Journal of Tropical Biomedicine, 2(1), S136–S139. https://doi.org/10.1016/S2221-1691(12)60144-0
Sudarsono, D. Gunawan, S. Wahyuono, I.A.Donatus. & Purnomo. 2002. Tumbuhan Obat II: Hasil Penelitian, Sifat-Sifat, dan Penggunaan. Yogyakarta: Pusat Studi Obat Tradisional UGM.
Suryani, N., Nurjanah, D., & Indriatmoko, D. D. (2019). Aktivitas Antibakteri Ekstrak Batang Kecombrang (Etlingera elatior (Jack) R.M.Sm.) Terhadap Bakteri Plak Gigi Streptococcus mutans. Jurnal Kartika Kimia, 2(1), 23–29. https://doi.org/10.26874/jkk.v2i1.19
Suryati, N., Bahar, E., & Ilmiawati. (2017). Uji Efektivitas Antibakteri Ekstrak Aloe vera Terhadap Pertumbuhan Escherichia coli Secara In Vitro. Jurnal Kesehatan Andalas, 6(3), 518–522. https://doi.org/10.25077/jka.v6i3.732
Tiwari, P., Kumar, B., Mandeep, K., Kaur, G., & Kaur, H. (2011). Phytochemical screening and Extraction: A Review. Internationale Pharmaceutica Sciencia, 1(1), 98–106.
Tjitda, P. J. . P., & Nitbani, F. O. (2019). SKRINING FITOKIMIA EKSTRAK METANOL, KLOROFORM DAN N-HEKSAN DAUN FLAMBOYAN. Sains Dan Terapan Kimia, 13(2), 70–79.
Tohomi, K. L., Iswahyudi, & Wahdaningsih, S. (2014). Aktivitas Antioksidan Ekstrak Etanol Daun Buas-Buas (Premna cordifolia Linn.) terhadap Gambaran Histopatologi Paru Tikus (Rattus norvegicus) Wistar Jantan Pasca Paparan Asap Rokok. Journal of Tropical Pharmacy and Chemistry, 2(4), 212–224.
Utomo, S. B., Fujiyanti, M., Lestari, W. P., & Mulyani, S. (2018). Uji Aktivitas Antibakteri Senyawa Hexadecyltrimethylammonium-Bromide Terhadap Bakteri Staphylococcus aureus dan Escherichia coli Antibacterial Activity Test of the C-4-methoxyphenylcalix [ 4 ] resorcinarene Compound Modified by Hexadecyltrimethylammonium-. Kimia Dan Pendidikan, 3(3), 201–209.
Vadivu, R., Suresh, A. J., Girinath, K., Kannan, P. B., Vimala, R., & Kumar, N. M. S. (2009). Evaluation of Hepatoprotective and In-vitro Cytotoxic Activity of Leaves of Premna serratifolia Linn. Journal Of Scientific Research, 1(1), 145–152. https://doi.org/10.3329/jsr.vlil.1046
Verdiana, M., Widarta, I. W. R., & Permana, I. D. G. M. (2018). Pengaruh jenis pelarut pada ekstraksi menggunakan gelombang ultrasonik terhadap aktivitas antioksidan ekstrak kulit buah lemon (Citrus limon (Linn) Burm F.). Jurnal Ilmu Dan Teknologi Pangan (ITEPA), 7(4), 213–222. https://doi.org/10.24843/itepa.2018.v07.i04.p08
Wikananda, I., Hendrayana, M., & Pinatih, K. (2019). Efek antibakteri ekstrak ethanol kulit batang tanaman cempaka kuning (M. champaca L.) terhadap pertumbuhan Staphylococcus aureus. E-Jurnal Medika, 8(5).